Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin

• Jon Lundstrøm,
• Emilie Gillon,
• Valérie Chazalet,
• Nicole Kerekes,
• Antonio Di Maio,
• Ten Feizi,
• Yan Liu,
• Annabelle Varrot and
• Daniel Bojar

Beilstein J. Org. Chem. 2024, 20, 306–320, doi:10.3762/bjoc.20.31

• : Supplementary glycan microarray document (MIRAGE) for the ICL glycan arrays. Acknowledgements We acknowledge support from the Mammalian Protein Expression core facility at the University of Gothenburg via the Protein Production Sweden (PPS) framework as well as the synchrotron SOLEIL (Saint Aubin, France) for

• glycan microarray studies were performed in the Carbohydrate Microarray Facility at the ICL Glycosciences Laboratory, which is supported by Wellcome Trust biomedical resource grants (099197/Z/12/Z, 108430/Z/15/Z, and 218304/Z/19/Z) and partially by the March of Dimes Prematurity research centre grant (22

Tools for generating and analyzing glycan microarray data

• Akul Y. Mehta,
• Jamie Heimburg-Molinaro and
• Richard D. Cummings

Beilstein J. Org. Chem. 2020, 16, 2260–2271, doi:10.3762/bjoc.16.187

• the mammalian body. They play a vital role in a number of physiologic and pathologic conditions. Glycan microarrays allow a plethora of information to be obtained on protein–glycan binding interactions. In this review, we describe the intricacies of the generation of glycan microarray data and the

• experimental methods for studying binding. We highlight the importance of this knowledge before moving on to the data analysis. We then highlight a number of tools for the analysis of glycan microarray data such as data repositories, data visualization and manual analysis tools, automated analysis tools and

• structural informatics tools. Keywords: data analysis; glycan binding; glycan microarray; glycomics; informatics; Introduction Glycans represent a major type of biomolecule in all living things, along with DNA, RNA, lipids and proteins [1]. In mammals, glycans commonly occur as post-translational

De novo synthesis of D- and L-fucosamine containing disaccharides

• Daniele Leonori and
• Peter H. Seeberger

Beilstein J. Org. Chem. 2013, 9, 332–341, doi:10.3762/bjoc.9.38

• (four steps, two chromatographic purifications) that after the direct oxidation–dihydroxylation–peracetylation protocol yielded the desired L-fucosamine building block L-8a (Scheme 5). Synthesis of fucosamine-containing disaccharides To facilitate the use of the bacterial monosaccharides in the glycan

• microarray platform and their conjugation to carrier proteins, an appropriate linker was required at the anomeric position [67]. Placement of a C3 naphthyl ether anticipated the site for further glycosylation at this position, which typically serves as the connection to the next sugar. Therefore, building